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mouse surfactant protein d elisa kit  (Beijing Solarbio Science)


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    Beijing Solarbio Science mouse surfactant protein d elisa kit
    An Increase in Circulating mtDNA Is Associated with Inflammation and Necroptosis in Septic ALI. ( A ) Representative H&E-stained photomicrographs of murine lung tissues from the control (0 h), 6 h, 12 h, and 24 h groups. Bar = 25 μm (original magnification ×100, ×400). ( B ) Lung injury scores for the control (0 h), 6 h, 12 h, and 24 h groups. ( C ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) measured by <t>ELISA</t> in mice after CLP at 0, 6, 12, and 24 h. ( D-I ) Correlations between serum mtDNA levels and lung injury biomarkers (SP-D and sRAGE) assessed using the Spearman correlation test. mtDNA levels were analyzed via quantitative real-time PCR using three mtDNA primers (D-loop, mt-COX1, mMito). ( J ) Western blot analysis of necroptosis signaling in murine lung tissues at 0, 6, 12, and 24 h after CLP. ( K ) Quantification of Western blot results. Data are presented as mean ± SD from at least four independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test
    Mouse Surfactant Protein D Elisa Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+surfactant+protein+d+elisa+kit/Mouse+C-Reactive+Protein+ELISA+KIT-96T/pmc12057123-19-0-7
    Average 93 stars, based on 5 article reviews
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    1) Product Images from "Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release"

    Article Title: Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release

    Journal: Molecular Medicine

    doi: 10.1186/s10020-025-01228-z

    An Increase in Circulating mtDNA Is Associated with Inflammation and Necroptosis in Septic ALI. ( A ) Representative H&E-stained photomicrographs of murine lung tissues from the control (0 h), 6 h, 12 h, and 24 h groups. Bar = 25 μm (original magnification ×100, ×400). ( B ) Lung injury scores for the control (0 h), 6 h, 12 h, and 24 h groups. ( C ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) measured by ELISA in mice after CLP at 0, 6, 12, and 24 h. ( D-I ) Correlations between serum mtDNA levels and lung injury biomarkers (SP-D and sRAGE) assessed using the Spearman correlation test. mtDNA levels were analyzed via quantitative real-time PCR using three mtDNA primers (D-loop, mt-COX1, mMito). ( J ) Western blot analysis of necroptosis signaling in murine lung tissues at 0, 6, 12, and 24 h after CLP. ( K ) Quantification of Western blot results. Data are presented as mean ± SD from at least four independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test
    Figure Legend Snippet: An Increase in Circulating mtDNA Is Associated with Inflammation and Necroptosis in Septic ALI. ( A ) Representative H&E-stained photomicrographs of murine lung tissues from the control (0 h), 6 h, 12 h, and 24 h groups. Bar = 25 μm (original magnification ×100, ×400). ( B ) Lung injury scores for the control (0 h), 6 h, 12 h, and 24 h groups. ( C ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) measured by ELISA in mice after CLP at 0, 6, 12, and 24 h. ( D-I ) Correlations between serum mtDNA levels and lung injury biomarkers (SP-D and sRAGE) assessed using the Spearman correlation test. mtDNA levels were analyzed via quantitative real-time PCR using three mtDNA primers (D-loop, mt-COX1, mMito). ( J ) Western blot analysis of necroptosis signaling in murine lung tissues at 0, 6, 12, and 24 h after CLP. ( K ) Quantification of Western blot results. Data are presented as mean ± SD from at least four independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

    Techniques Used: Staining, Control, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot

    Melatonin Mitigates Septic ALI by Reducing Circulating mtDNA Release and Inflammatory Injury. ( A ) Survival rates of mice with or without melatonin therapy 24 h after CLP (log-rank [Mantel-Cox] test, n = 20). ( B ) Lung wet/dry ratio in mice with or without melatonin therapy at 24 h after CLP. ( C ) BALF protein levels in mice with or without melatonin therapy at 24 h after CLP. ( D ) Representative H&E-stained photomicrographs of lung tissues from Sham, Sham + Mel, CLP, and CLP + Mel groups. Bar = 25 μm (original magnification ×100, ×400). ( E ) Lung injury scores from the Sham, Sham + Mel, CLP, and CLP + Mel groups. ( F-J ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in Sham, Sham + Mel, CLP, and CLP + Mel groups. ( K-O ) BALF levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in the same groups. ( P-R ) qPCR analysis of mt-Col, mt-Dloop, and mMito mRNA levels in indicated tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test
    Figure Legend Snippet: Melatonin Mitigates Septic ALI by Reducing Circulating mtDNA Release and Inflammatory Injury. ( A ) Survival rates of mice with or without melatonin therapy 24 h after CLP (log-rank [Mantel-Cox] test, n = 20). ( B ) Lung wet/dry ratio in mice with or without melatonin therapy at 24 h after CLP. ( C ) BALF protein levels in mice with or without melatonin therapy at 24 h after CLP. ( D ) Representative H&E-stained photomicrographs of lung tissues from Sham, Sham + Mel, CLP, and CLP + Mel groups. Bar = 25 μm (original magnification ×100, ×400). ( E ) Lung injury scores from the Sham, Sham + Mel, CLP, and CLP + Mel groups. ( F-J ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in Sham, Sham + Mel, CLP, and CLP + Mel groups. ( K-O ) BALF levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in the same groups. ( P-R ) qPCR analysis of mt-Col, mt-Dloop, and mMito mRNA levels in indicated tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

    Techniques Used: Staining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

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    Article Title: Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release
    Article Snippet: Mouse TNF-α ELISA KIT , Solarbio , SEKM-0034. .. Mouse Surfactant protein D ELISA KIT , Solarbio , SEKM-0196. .. Mouse RAGE ELISA KIT , Solarbio , SEKM-0128.



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    Image Search Results


    An Increase in Circulating mtDNA Is Associated with Inflammation and Necroptosis in Septic ALI. ( A ) Representative H&E-stained photomicrographs of murine lung tissues from the control (0 h), 6 h, 12 h, and 24 h groups. Bar = 25 μm (original magnification ×100, ×400). ( B ) Lung injury scores for the control (0 h), 6 h, 12 h, and 24 h groups. ( C ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) measured by ELISA in mice after CLP at 0, 6, 12, and 24 h. ( D-I ) Correlations between serum mtDNA levels and lung injury biomarkers (SP-D and sRAGE) assessed using the Spearman correlation test. mtDNA levels were analyzed via quantitative real-time PCR using three mtDNA primers (D-loop, mt-COX1, mMito). ( J ) Western blot analysis of necroptosis signaling in murine lung tissues at 0, 6, 12, and 24 h after CLP. ( K ) Quantification of Western blot results. Data are presented as mean ± SD from at least four independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

    Journal: Molecular Medicine

    Article Title: Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release

    doi: 10.1186/s10020-025-01228-z

    Figure Lengend Snippet: An Increase in Circulating mtDNA Is Associated with Inflammation and Necroptosis in Septic ALI. ( A ) Representative H&E-stained photomicrographs of murine lung tissues from the control (0 h), 6 h, 12 h, and 24 h groups. Bar = 25 μm (original magnification ×100, ×400). ( B ) Lung injury scores for the control (0 h), 6 h, 12 h, and 24 h groups. ( C ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) measured by ELISA in mice after CLP at 0, 6, 12, and 24 h. ( D-I ) Correlations between serum mtDNA levels and lung injury biomarkers (SP-D and sRAGE) assessed using the Spearman correlation test. mtDNA levels were analyzed via quantitative real-time PCR using three mtDNA primers (D-loop, mt-COX1, mMito). ( J ) Western blot analysis of necroptosis signaling in murine lung tissues at 0, 6, 12, and 24 h after CLP. ( K ) Quantification of Western blot results. Data are presented as mean ± SD from at least four independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

    Article Snippet: Mouse Surfactant protein D ELISA KIT , Solarbio , SEKM-0196.

    Techniques: Staining, Control, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot

    Melatonin Mitigates Septic ALI by Reducing Circulating mtDNA Release and Inflammatory Injury. ( A ) Survival rates of mice with or without melatonin therapy 24 h after CLP (log-rank [Mantel-Cox] test, n = 20). ( B ) Lung wet/dry ratio in mice with or without melatonin therapy at 24 h after CLP. ( C ) BALF protein levels in mice with or without melatonin therapy at 24 h after CLP. ( D ) Representative H&E-stained photomicrographs of lung tissues from Sham, Sham + Mel, CLP, and CLP + Mel groups. Bar = 25 μm (original magnification ×100, ×400). ( E ) Lung injury scores from the Sham, Sham + Mel, CLP, and CLP + Mel groups. ( F-J ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in Sham, Sham + Mel, CLP, and CLP + Mel groups. ( K-O ) BALF levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in the same groups. ( P-R ) qPCR analysis of mt-Col, mt-Dloop, and mMito mRNA levels in indicated tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

    Journal: Molecular Medicine

    Article Title: Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release

    doi: 10.1186/s10020-025-01228-z

    Figure Lengend Snippet: Melatonin Mitigates Septic ALI by Reducing Circulating mtDNA Release and Inflammatory Injury. ( A ) Survival rates of mice with or without melatonin therapy 24 h after CLP (log-rank [Mantel-Cox] test, n = 20). ( B ) Lung wet/dry ratio in mice with or without melatonin therapy at 24 h after CLP. ( C ) BALF protein levels in mice with or without melatonin therapy at 24 h after CLP. ( D ) Representative H&E-stained photomicrographs of lung tissues from Sham, Sham + Mel, CLP, and CLP + Mel groups. Bar = 25 μm (original magnification ×100, ×400). ( E ) Lung injury scores from the Sham, Sham + Mel, CLP, and CLP + Mel groups. ( F-J ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in Sham, Sham + Mel, CLP, and CLP + Mel groups. ( K-O ) BALF levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in the same groups. ( P-R ) qPCR analysis of mt-Col, mt-Dloop, and mMito mRNA levels in indicated tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

    Article Snippet: Mouse Surfactant protein D ELISA KIT , Solarbio , SEKM-0196.

    Techniques: Staining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay